With the introduction of the Robot Operating System (ROS), the industry has now seen a shift in the way startups are turning to robotics, automation, and the digital innovation to gain agility and scalable expansion. Although it has global applicability, little attention has been paid in comprehending the effect of ROS adoption on startup agility and scaling, especially in the up-and-coming economy like Nigeria. The theoretical frameworks that will be discussed in this conceptual paper to identify the impact of ROS on the startup agility and scalability are the Resource-Based View (RBV) and the Dynamic Capabilities Theory. The research design adopted in the study is a conceptual research design that wholly relies on secondary data that has been obtained through journals, conference proceedings, books, and technical reports. It has been found that ROS increases modularity, interoperability and containerized deployment, promotes agility, scalability and innovation in startup ecosystems. In addition, ROS is a resource that is dynamic and can be reconfigured flexibly and distributed architecture, enhancing operational effectiveness and entrepreneurial performance. Some of the recommendations made in the paper include creation of ROS-based training, creation of industry academia partnerships, and creation of policy frameworks that will guide the open-source robotics use by startups and SMEs. The study concludes by confirming that the strategic execution of ROS can serve as an impetus to agile and scalable start-ups.