The purpose of this study was to determine the level of selected antioxidant enzymes, CD4+ cell count and Uric in HIV seropositive individual attending Federal Medical Centre Owo, Ondo State. Glutathione peroxidase, catalase and superoxide dismutase levels were determined in one hundred and fifty subjects comprising, fifty [50] seropositive subject on anti-retroviral therapy, fifty [50] seropositive subject not on anti-retroviral therapy and fifty [50] seronegative subject, the control group using spectrophotometry method. The result shows that the mean catalase activities of the subjects when compared with that of HIV patients without ART was statistically significant (p<0000), mean catalase activities between HIV patient on ART and those without art were also highly significant. Catalase activity in the sampled showed no significant correlation with CD4-cell count (p>0.05). SOD activities in HIV patient on ART and those without ART were higher than the control subjects, but not statistically significant (p>0.05). Uric acid level showed a highly significant increase in both sero positive patients when compared with control. (p>000). Glutathione peroxidase activity in HIV patient without ART and those on ART was found to be significantly higher (p-value) when activity in the control subject, (p<0.000) significant CD4 depletion was observed in HIV patient without the ART treated patients, SOD positively correlates with CD4+ cell count (p<0.05) showing protective role of SOD against oxidative CD4+ cells destruction. In conclusion, the oxidant capacity of HIV patients without HAART confirmed the involvement of oxidative stress in lymphocyte destruction of HIV in these subjects. Oxidative stress will amplify the impact of the adverse effects of HIV infection in premature death of immune cells. The level of antioxidant enzymes in our study population is now known. The study created an avenue for the establishment of the relationship between HIV and antioxidant enzymes (CAT, SOD, GPX) Uric acid and CD4+ cell counts.