Malaria is a parasitic infectious vector-borne disease and major public’s health problem with haematological parameters. The Aim of this research was to determine the effect of Carica papaya leaves on Plasmodium falciparum. The antiplasmodial activity of the C. papaya was performed in 96-well tissue culture plates. Serial dilutions of test samples dissolved in sterile methanol were placed in microtiter plates and diluted with culture medium. Parasitized erythrocytes were added to the wells to give a final volume of 110µl. Chloroquine was used as standard. The plates were incubated at 37oC with 5%CO2. After incubation for 24 hours, Giemsa-stained thin blood films were prepared and the percentage of inhibition of parasite was determined under microscope. The percentage inhibition at each concentration was determined and the mean calculated. At 100 (µg/ml) the water based extract, ethanol extract and Chloroquine standard produced a mean of 0.00, 0.00 and 0.62 parasitaemia and at 50 (µg/ml) the water based extract, ethanol extract and Chloroquine produced a mean of 0.37, 0.34 and 0.62. While at Concentration level of 25 (µg/ml) the water based extracts and ethanol extract produced a mean of 1.05, 0.93 and 0.62. At 12.5 (µg/ml) the water based extract, ethanol extract and chloroquine produced a mean of 1.25, 1.06 and 0.62 respectively against the control 3.5. This trend signifies a decline in Plasmodium falciparum proliferations as the concentration increases from 12.5 to 100 (µg/m L). Also ethanol based extract have more anti-malarial effect on P. falciparum followed by water base extract at 100 (µg/m L). The Government should support research and provide vaccine against malarial parasites for its citizen.

Keywords: Invitro, Carica papaya leaves, plasmodium falciparum, antiplasmodial activity.